ANEX,Rat,SHR,WKY

Matrix Data for SHR, SHRSP and WKY Strains


At least more than 20 biochemical marker loci in each line were screened and number of different alleles in the loci was compared by Matrix computer programs made by Dr. T. Yoshinaga, University of Tokushima. This programs can discriminate strain difference and/or similarity among more than 1000 strains.
Now I have huge Matrix data, but it is impossible to display all here, so that I would like to show you first about SHR and related strains. From the data, much difference is not observed in lines derived from same group of SHR or SHRSP. However, many allele differences are observed in WKY group. Of course, much differences are observed as compared to group to other groups.

Many people questioned that WKY was real control rat for SHR or SHRSP. Approximately 15 years ago, most people didn't know any genetic differences among rat strains, because no one had good procedure to analyze them genetically. So, they only believed that those lines were kept by brother-sister mating without strain contamination, and recorded it for a long time. Another words, the breeding history was most important tool to explain that those strains were originated from real origin.

Yes, origin of SHR is WKY (Kyoto University) which was also originated from W/Hok (Hokkaido University), very long time ago. This is evident because W/Hok, WKY(some case, not all WKY, anyway) and SHR share RT1k allele which is very rare in laboratory rat in the world. There are several other minor finding that these strains are originated form same original rat. Now it is not clear what happen in WKY during Kyoto University, or in W/Hok, but it was real that the WKY lines were heterozygous population at that time, and distributed to the world.

However, it is not so easy to say what type of strain you call as control line is real control. It seems that WKY has advantage in the traits as control line of SHR than the other strains. In this meaning, WKY should be one of control lines of SHR. But in another fields, it is not always way, especially, strains far from SHR are better in genetic analyses. In general, I feel that it is not necessary to be particular about the control line. If you need real control, it would be nice to establish congenic lines to F344 or other background strains.


Matrix DATA for SHR, SHRSP and WKY

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                                                                                               Number of 
                   1  2  3  4  5  6  7  8  9   10 11 12 13 14 15 16   17 18 19 20 21 22 23 24  loci tested
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 1   SHR/Cpb 1)    0  0  0  1  0  1  0  2  1    1  3  2  3  3  2  3    9 10  5  7  8 10 10  9      24
 2   SHR/Han 1)    0  0  0  0  0  1  1  1  1    0  2  1  2  2  1  2    8  9  5  7  7 10 10  9      22
 3   SHR/Ici 1)    0  0  0  0  0  0  0  1  0    1  3  2  2  2  2  2    7  8  4  6  6  7  8  8      18
 4   SHR/Kyo 1)    1  0  0  0  1  0  1  1  0    2  4  3  4  4  3  4    9 10  4  6 10  9 12 10      27
 5   SHR/NCrj      0  0  0  1  0  2  1  3  2    1  3  2  3  3  2  3    7  8  3  5 10  9  9  7      23
 6   SHRSRCL/Izm   1  1  0  0  2  0  1  1  0    4  4  5  6  6  5  6    9 11  5  7 12 11 13 11      30
 7   SHRSRB1/Izm   0  1  0  1  1  1  0  2  1    3  3  4  5  5  4  5    9 11  6  8 11 12 12 10      30
 8   SHRSRB2/Izm   2  1  1  1  3  1  2  0  1    3  3  4  5  5  4  5    8 10  6  8 11 12 12 10      30
 9   SHRSRCH/Izm   1  1  0  0  2  0  1  1  0    4  4  5  6  6  5  6    9 11  5  7 12 11 13 11      30
                                                                                              
10   SHRSP/Kur     1  0  1  2  1  4  3  3  4    0  1  0  0  0  0  0    7  7  7  9  6 10  9  8      24
11   SHRSP/Rm 1)   3  2  3  4  3  4  3  3  4    1  0  1  2  2  1  2    8  7  5  7  5  9  7  6      24
12   SHRSP/N       2  1  2  3  2  5  4  4  5    0  1  0  1  1  0  1    9  8  7  9  7 11 10  8      29
13   SHRSPA1/Izm   3  2  2  4  3  6  5  5  6    0  2  1  0  0  0  0    8  7  7  9  7 11  9  9      29
14   SHRSPA3/Izm   3  2  2  4  3  6  5  5  6    0  2  1  0  0  0  0    8  7  7  9  7 11  9  9      30
15   SHRSPA3H/Izm  2  1  2  3  2  5  4  4  5    0  1  0  0  0  0  0    8  7  7  9  7 11  9  8      29
16   SHRSPA4/Izm   3  2  2  4  3  6  5  5  6    0  2  1  0  0  0  0    8  7  7  9  7 11  9  9      29
                                                                                              
17   WKY/Cpb 1)    9  8  7  9  7  9  9  8  9    7  8  9  8  8  8  8    0  1  7  8  1  2  2  2      23
18   WKY/Han 1)   10  9  8 10  8 11 11 10 11    7  7  8  7  7  7  7    1  0  8  9  0  2  2  2      23
19   WKY/Kur1      5  5  4  4  3  5  6  6  5    7  5  7  7  7  7  7    7  8  0  3  8  6  8  8      25
20   WKY/Kur2      7  7  6  6  5  7  8  8  7    9  7  9  9  9  9  9    8  9  3  0 10  7 11  9      25
21   WKY/NCrj      8  7  6 10 10 12 11 11 12    6  5  7  7  7  7  7    1  0  8 10  0  3  1  3      24
22   WKY/Izm      10 10  7  9  9 11 12 12 11   10  9 11 11 11 11 11    2  2  6  7  3  0  5  4      30
23   WKYO/Ham     10 10  8 12  9 13 12 12 13    9  7 10  9  9  9  9    2  2  8 11  1  5  0  3      30
24   WKYO/Kyo      9  9  8 10  7 11 10 10 11    8  6  8  9  9  8  9    2  2  8  9  3  4  3  0      30
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Most of data were screened by us using biochemical markers .


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